To investigate the specific function of ALKBH5 in the progression of SKCM, we transfected cells with lentiviruses packaged with distinct shRNA sequence plasmids (shALKBH5#1, shALKBH5#2, and shALKBH5#3) and constructed stable ALKBH5-knockdown cells in melanoma A375 and A2058 cell lines. Here, ALKBH5 is linked to melanoma.