DNMT3A and glioblastoma: More importantly, mTORC2 (Rictor)-dependent shift in global DNA methylation level surrogated by LINE-1 methylation was rescued by the concurrent inhibition of DNMT3A (Fig. 4C), and the S473 phosphorylation of Akt (mTORC2 activation marker), loss of DNMT3A as well as 5-mC are correlated in human GBM samples (Fig. 4D).