<h4>Background</h4>Pathogenic variants of the IRF2BPL gene have been reported to cause neurodevelopmental disorders; however, studies focused on IRF2BPL in zebrafish are limited.<h4>Results</h4>We reported three probands diagnosed with developmental delay and epilepsy and investigated the role of IRF2BPL in neurodevelopmental disorders in zebrafish. This evidence concerns the gene IRF2BPL and neurodevelopmental disorder.