To investigate the transcriptional state of postmortem human AD brain tissue in relationship to the APOE genotype we performed single-nucleus RNA sequencing (snRNA-seq) on fresh-frozen frontal cortex tissue from individuals diagnosed with AD with the APOE4/4 genotype, individuals with AD and an APOE3/3 genotype and age and sex-matched control individuals with the APOE3/3 genotype (Fig. 1a and Supplementary Table 1). This evidence concerns the gene APOE and Alzheimer disease.