For NR1D1, the average AOD value of RB tissues is 0.243 ± 0.031, which is significantly lower than the average AOD of 0.276 ± 0.043 in normal retina (P = 0.004), while the AOD of NR1D1 in the adjacent non-tumor retina of RB patients (0.256 ± 0.036) was not significantly different from the AOD of normal retina (P = 0.108) but higher than that of RB tissue (P = 0.041) (Fig. 3a). This evidence concerns the gene NR1D1 and retinoblastoma.