CASP3 and neoplasm: Furthermore, we explored Caspase-3 and the proliferation marker Ki67 in these tumors by IHC, finding a 2–3 fold increase in the number of active Caspase-3 positive cells (Fig. 5H), and a decrease in the proportion of Ki67-positive cells (Fig. 5I) in sh conditions, which confirmed in vivo the requirement of ANKRD6 for tumor cell survival and proliferation.