The eight-gene predictive model showed a remarkable capacity for calibration and discrimination in predicting AD, suggesting that CARTPT, KLHL35, NRN1, NT5DC3, PCYOX1L, RHOQ, SLC6A12 and SLC38A2 have the potential to serve as diagnostic markers for AD. Furthermore, the qRT-PCR, WB and IHC experiments using brain tissues also confirmed the overexpression of RHOQ and SLC6A12 and the downregulation of CARTPT, KLHL35, NRN1, NT5DC3 and PCYOX1L in AD mice compared to controls. Here, SLC6A12 is linked to Alzheimer disease.