CD8A and neoplasm: We used enzyme‐linked immunospot (ELISpot) assay to detect tumor antigen‐specific, IFN‐γ secreting CD8+ T cells in the spleens.[45] Splenocytes from treated MC38 tumor‐bearing mice were stimulated with KSPWFTTL (KSP) peptide and the IFN‐γ secreting spot‐forming cells (SFCs) were quantified.