Through combining multiple sEV cargos, an HCC EV ECG score, which is an EV-based protein assay and calculated from the readouts of three HCC EV subpopulations (EpCAM+CD63+, CD147+CD63+, and GPC3+CD63+ EVs), was established for detecting early-stage HCC (83). Here, CD63 is linked to hepatocellular carcinoma.