The sEVs derived from these CD133+ HCCs, as compared to the sEVs secreted by the CD133- cells, were shown to promote migration, proliferation, self-renewal, and epithelial-mesenchymal transition (EMT) of target non-CSC HCC cells in vitro, via transfer of circ-ZEB1 and circ-AFAP1 (8). This evidence concerns the gene PROM1 and hepatocellular carcinoma.