In overall, the 11 immune cell subsets in melanoma included memory B cells, naïve B cells, resting Mast cells, and M0, M1, M2 macrophages, resting NK cells, CD 8 T cells, follicular helper T cells, regulatory Tregs T cells and CD4 memory T cells have been activated, whose sum of mean proportions was more than 75% in all melanoma samples (Figure 6B). This evidence concerns the gene CD4 and melanoma.