One attempt at creating a synthetic polymeric biomaterial scaffold involved scaffolds prepared using E-jet 3D printing of PLGA seeded with HCT-116 and LoVo human colon cancer cell lines, as well as the p53-null (knockout) human colon cancer cell line (HCT-116 p53−/−) [124]. The gene discussed is TP53; the disease is malignant colon neoplasm.