RT-qPCR and Western blot analysis of P1 cerebral hemispheres in the high FA group showed sex-specific changes in transcription factors Nfix, Runx1, and Vgll2, DNA Methyltransferase Dnmt3b, the imprinted gene Dio3, H19, and Xist, and the candidate autism susceptible gene Auts2, Fmr1 at mRNA levels, and Gad1, Park2, and Hsp90 at protein levels. The gene discussed is NFIX; the disease is autism.