Unlike current PCLSs models, which show only steatosis, lipid deposition, and lipotoxicity [10,11,12,38], human PCLSs under GFIPO after 96 h exhibited markers of liver inflammation, including up-regulated inflammatory genes (TNFα, IL6, and IL1β) and cytokines (TNFα, IL6, IL8), detectable up to 96 h. The gene discussed is TNF; the disease is steatosis.