SIRT1 and colorectal cancer: For example, screening of a nicotinamide and benzamide library identified a 2-anilinobenzamide compound as a modest Sirt1 inhibitor with ~4 and ~14-fold selectivity for Sirt1 over Sirt2 and Sirt3 [207]; cellular activity was confirmed by a concentration-dependent increase in acetylation of the Sirt1 substrate p53 in the HCT116 colorectal cancer cell line [207].