Our data indicate that B7-H3 binding in a classical Fc non-silenced antibody format showed low impact on the viability of tumor cells; only the addition of a CD16 or both a CD16 and TIGIT Fab led to the increased cytotoxic potential of NK cells, corroborating the notion that more complex formats with carefully adjusted tumor and NK cell binding modules are required for a strong anti-tumor activity. This evidence concerns the gene FCGR3A and neoplasm.