Although in vitro experiments have shown enhanced results for both MDA-MB-231 and 4T1 cells, which both have VEGF expression and have been used for VEGF-related experiments, we proceeded to animal studies due to the fact that 4T1 is a very fast-growing cancer cell line with high vascularization and tumors are formed quickly [59,60,61]. This evidence concerns the gene VEGFA and cancer.