In the present study, colonic TLR4 expression, phosphorylation levels of NF-κB (p65 and IκB), and MAPK (p38, ERK1/2, and JNK1/2) signaling pathway proteins were markedly elevated in mice with colitis compared with the control group, while Amy administration markedly decreased the TLR4 expression and phosphorylation levels of these pathway proteins. Here, MAPK3 is linked to colitis.