Furthermore, when the analyses considered potential sex differences, it was found that female adolescents with MDD only had increased levels of IL-10 and TNF-α, while male adolescents with MDD had increased levels of IL-4, IL-10, sIL-6R, Treg + Th2, and TNF-α/TNF-R1 [191]. This evidence concerns the gene TNF and major depressive disorder.