First, we compared the levels of two autophagosome markers, microtubule-associated protein 1A/1B-light chain 3 (LC3) and p62, on the MELHO wild-type melanoma cell line and in the three RUNX2 KO cell lines: 1E7, 1B3 and 1F5. The gene discussed is SQSTM1; the disease is melanoma.