In fact, the CD8+ CD28− CD27− LT subtype was increased in CS patients (30.48 ± 5.19) compared to healthy donors (18–29 years: 6.86 ± 9.02; p = 0.0023 and to 30–45 years: 14.30 ± 11.1; p = 0.024) (Figure 3f), while the CD8+ CD28+ CD27+ LT subtype was significantly decreased in CS patients (45.6 ± 5.23) compared to healthy young donors (18–29 years: 79.3 ± 15.08; p = 0.0081), and to elderly group (46–65 years: 48.48 ± 12.45; p = 0.029) (Figure 3e). Here, CD28 is linked to Cowden syndrome 1.