Besides NKCC2, AUP1 also binds to the structurally related cotransporter NCC and regulates its protein expression, suggesting that the interaction with AUP1 is a common feature of members of cation-chloride cotransporters, a group of proteins that are targets of therapeutic drugs and mutated in several human pathologies such as Gitelman, Bartter, and Andermann syndromes [11,63,64]. This evidence concerns the gene SLC12A3 and Corpus callosum agenesis - neuronopathy.