With the 20-color panel tube, the fifteen samples with neg MRD based on routine cytometry, patterns similar to normal BMs were found in the CD371/CD45RA dotplots, with populations present in the four quadrants, although with some differences; a decrease in CLPs was found in 2/15 samples and a decrease in HSP/MPP populations was found in 8/15 samples. This evidence concerns the gene CLEC12A and hereditary spastic paraplegia.