Our data in Figure S3A–C show that feeding an MCD diet resulted in comparable levels of liver fibrosis markers [PSR staining (6–6.5-fold), hydroxyproline levels (1.8-fold), and fibrosis markers (Col1α1, 4–5-fold and Col3α1, 2–2.5-fold)] in WT and Mlkl−/− mice compared to mice fed a methionine choline-sufficient diet (MSD). Here, COL1A1 is linked to Hepatic fibrosis.