ACTA1 and Hepatic fibrosis: The HE, Sirius Red, and IHC staining results demonstrated that LRRC1 inhibition through AAV–shLRRC1 effectively alleviated CCl4-induced liver fibrosis, as evidenced by lower Ishak scores, a reduced Sirius-Red-stained area, and decreased α-SMA IHC scores compared to those in the CCl4+AAV–empty group (Figure 5B–F).