By investigating the underlying mechanisms of C1QBP inhibition using its specific inhibitor M36, we not only confirmed the important role that p32/C1QBP plays in the positive modulation of oncogenic pathways downstream from RTKs but also found that C1QBP inhibition with M36 affects the crucial role of C1QBP in the maintenance of mitochondrial integrity and dynamics in colon cancer cells. Here, C1QBP is linked to colonic neoplasm.