Using the BC Subtyping Kit analysis software (https://www.apisassay.com/breast-cancer-subtyping, accessed on 7 January 2024) developed by APIS Assay Technologies (Manchester, UK), the results calling produced 76 samples that were ER-positive (above −1.98; the cut-off value), 65 samples that were PR-positive (above −0.63) and 14 samples that were HER-2-positive (above 2.00). This evidence concerns the gene ESR1 and breast cancer.