KRAS and Increased total leukocyte count: asTF-proficient, KRAS G12V-mutant cell line PaCa-44 yields reproducible-size, aggressive primary orthotopic tumors that spread spontaneously to PDAC-relevant anatomical sites (Figure 2); other useful features of the PaCa-44 model comprise its 100% penetrance of metastases to the site of surgical incision (wound closure area in the abdominal wall), and severe leukocytosis.