The same evaluation, of integrated density, was also conducted on the collagenolytic activities present in the extracts (see M&M) of NAM in the co-culture, treated with the CSF of AD subjects (Aβ+Tau and Aβ-Tau) and non-AD subjects (non-AD), and developed in the presence of CaCl2 or EDTA. The gene discussed is MAPT; the disease is Alzheimer disease.