MAPT and Alzheimer disease: We, therefore, analyzed, both in the CM and in the extracts from NAM co-cultures treated with CSF from AD subjects (groups Aβ+tau and Aβ-tau, together) and non-AD patients (group non-AD), the presence of gelatinolytic activities via zymography developed in the presence of CaCl2 (activator of MMPs and other Ca2+-dependent proteolytic enzymes) or EDTA.