Western blot analysis also confirmed that β-catenin, c-Myc, and cyclin D1 proteins are highly expressed in GFAP-Cre; KrasG12D; APCL/+; p53L/L cells compared to GFAP-Cre; KrasG12D; p53L/L cells (Figure 4B), Our data suggested that the inactivation of APC in GBM may lead to the aberrant activation of the WNT/β-catenin signaling pathway to promote GBM progression. This evidence concerns the gene GFAP and glioblastoma.