Tumorigenicity refers to the capacity of a cell or cell population to form tumors, and our result suggests that APC haploinsufficiency has a more pronounced effect on the tumorigenic potential of GBM cells, influencing their ability to grow and form tumor-like structures, which suggests that APC haploinsufficiency not only influences the rate of cell proliferation but also plays a significant role in promoting the tumorigenicity of GBM cells. Here, APC is linked to glioblastoma.