A role for the participation of IF1 in metabolic reprogramming of the cells was ruled out because it was found that after silencing IF1 in 143B osteosarcoma, HCT116 colon cancer, and HeLa cervix adenocarcinoma cell lines, the rates of ATP synthesis, basal cellular respiration, and mitochondrial membrane potential (ΔΨm) resulted in no relevant changes [1]. Here, ATP5IF1 is linked to osteosarcoma.