In 38 eligible samples to ddPCR,ITEC (IDH1-pTERT-EGFR-CDKN2A) protocol was successfully performed in 26 cases (68.4%), and successfully diagnosed in 21 cases (55.3%).GBM IDH-wt 66.7% (20/32)Astrocytoma G4 50% (1/2)Astrocytoma G2/3 0% (0/2)Oligodendroglioma G2/3 0% (0/2). Here, CDKN2A is linked to astrocytoma (excluding glioblastoma).