Figure 4A,B show that NEO212 caused a prominent, persistent induction of both lytic cycle markers at concentrations as low as 1 μM, which was followed by increased levels of pro-apoptotic cleaved caspases 3 and 7. The induction of these lytic cycle proteins by NEO212 could also be documented in primary patient-derived BM-NPC cells (Figure 4C). In comparison, TMZ was unable to trigger the lytic cycle (Figure 4D). As a negative control, we used TW1 cells, and, as expected, no EBV proteins could be detected upon their treatment with NEO212 (Figure 4E). This evidence concerns the gene CASP3 and nasopharyngeal carcinoma.