In our study, we did not detect ROS and NLRP3, but higher levels of MDA, IL-1β, and IL-6 were found, as well as a sharp increase in caspase-1 levels and sperm cell apoptosis in diabetic rats, which also confirmed that oxidative stress and inflammation induced by diabetes can lead to the apoptosis of testicular cells. This evidence concerns the gene NLRP3 and diabetes mellitus.