In terms of its mechanism, PEBP4 deletion further reduced the protein expression of AFC-related markers including ENaC-α, ENaC-γ, Na,K-ATPase α1, and Na,K-ATPase β1, and enhanced the suppression of PI3K/AKT signaling pathway in ALI models, which were partially rectified by selective activation of PI3K/AKT pathways with 740YP or SC79. The gene discussed is AKT1; the disease is acute respiratory distress syndrome.