To evaluate if KO of MSUT2 alters markers of astrocyte or microglia activation after AD-tau seeding, we used immunohistology to assess astrocytes (GFAP) and microglia (Iba1) in the area of the injection site where glial activation can be observed in 7 d.p.i. mouse brains. The gene discussed is AIF1; the disease is Alzheimer disease.