We observed that the expression of KAL in the liver was also higher in MCD diet-induced NAFLD rats (Fig. 3d); Serpina4−/− rats showed a significant improvement in hepatic steatosis, inflammation, and collagen fiber deposition in MCD-induced NAFLD rats (Fig. 3e-f). The gene discussed is SERPINA4; the disease is metabolic dysfunction-associated steatotic liver disease.