When we fed KAL-Tg mice and wild-type (WT) mice with HFD, KAL-Tg mice exhibited extensive inflammation, injury, and fibrosis along with aggravated hepatic steatosis (Fig. 2p, supplementary Fig. 2j-m), and the expression of TNFα, interleukin-6 (IL-6), α-SMA, and collagen I was also increased (Fig. 2q-r, supplementary Fig. 2n). Here, ACTA1 is linked to fatty liver disease.