Since the 10-month-old mice did not exhibit extensive fibrosis with collagen deposition (supplementary Fig. 2f), we continued to raise the mice for 16-month-old and found that 16-month-old KAL-Tg mice developed not only NASH (supplementary Fig. 2g–i) but also hepatic fibrosis with elevated α-smooth muscle actin (α-SMA) and collagen I (Fig. 2m-o). Here, ACTA1 is linked to Hepatic fibrosis.