As expected, gD was present only in the Wt-infected cells – confirming that the ΔgD virus was of the expected phenotype – while VP16 expression was broadly similar in ΔgD and the parental Wt virus-infected cells, confirming that virus protein expression proceeded as normal during high multiplicity infection, even in the absence of gD (Fig. 5a). This evidence concerns the gene PAEP and infection.