Therefore, we obtained a PAO1 mutant strain in which the galU gene, encoding UGP, was disrupted by transposon insertion (galU−) (44) and compared its growth kinetics to WT PAO1 over 24 h in nutrient-rich lysogeny broth (LB) medium as well as the two cell culture media used in the infection studies described below. This evidence concerns the gene SMOX and infection.