Interestingly (and echoing what was observed with the less specific PMA/iono stimulation), there was, post PAO1, an increased production of neutrophilic granules components (S100A8/A9, lactoferrin, MMP-3, MMP-7, MMP-8, MMP-9, NE) in CF cells (Figures 8D, E, Supplementary Figure S11, and a similarly enhanced enzymatic activity of NE and metalloproteases (Figures 8F, G), correlated with the (maintained) high induction of G-CSF (a key regulator of neutrophil production) in CF PBMCs (Supplementary Figure S10K). This evidence concerns the gene ELANE and cystic fibrosis.