TNF and cystic fibrosis: When ‘pro-inflammatory’ mediators (TNF, IL-1b, IL-6, IL-8) were considered, PAO1 induced TNF, IL-1b, IL-6 mRNA in both HC and CF cells (Figures 7E–G), whereas IL-8 mRNA was poorly induced in CF PBMCs, compared to HC cells (Figure 7H).