Thirdly, PMA/iono up-regulated the mRNA of all these mediators, but the fold increase from the respective HC and CF controls (as assessed with the formula 2^(-ddCT) was invariably less pronounced in CF cells (medians of 1,093 v 8,027, 1.0 v 111, 133 v 6,122, 28.8 v 975 for IFN-g, IL-13, IL-17 and IL-22, respectively). This evidence concerns the gene IL22 and cystic fibrosis.