Indeed, RNA-seq analysis of FACS-sorted peritoneal CLL cells isolated from either TCL1-Tg or TCL1-Tg BAFF-/- mice showed differences in gene expression, which we ranked using the moderated t-statistics from a limma analysis (27) (Figure 6A), and by a supervised clustering approach involving a Partial Least Squares Discriminant Analysis (PLSDA) and sparse PLSDA (sPLSDA) (28) (Figure 6B). The gene discussed is TNFSF13B; the disease is B-cell chronic lymphocytic leukemia.