The OIA software is simple to use and install and has the following advantages: (1) it works with a photograph taken under a microscope and not with a scan of the glass; (2) it allows monitoring, i.e., to see the cells to be accounted for each category before image quantification; and (3) it allows to divide the epithelial strata into three thirds (basal/medium/superior), to compare the positivity of ki67 among layers and to study its relationship with the degree of epithelial dysplasia. This evidence concerns the gene MKI67 and intraepithelial neoplasia.