These results were further validated using an animal model of ischemic stroke, the middle cerebral artery occlusion (MCAo) model, where animals treated with the combination of progesterone and the let-7i antagomir showed a robust increase in mature-BDNF protein levels, significantly reduced infarct sizes, enhanced SYP expression, and improved functional recovery compared to vehicle-treated animals (118). The gene discussed is BDNF; the disease is ischemic stroke.