Intriguingly, GTPBP2 ablation significantly altered the proliferation, chemoresistance, and self-renewal of CCSCs rather than CD133−CD44− (Subset I) and CD133−CD44+ (Subset II) CRC cells, implying that GTPBP2 is essential for CCSC activity but not differentiated CRC cells. This evidence concerns the gene GTPBP2 and colorectal carcinoma.