PRAME is a cancer testis antigen (CTA) that is expressed in germline tissues, but not differentiated tissues, and has been investigated as a target for cancer immunotherapy in extracranial pediatric malignancies.41 Similarly, expression of gamete-specific genes including DAZL has been reported upon deletion of DNA methyltransferases in murine embryonic stem cells, consistent with our findings of its upregulation upon decitabine-induced hypomethylation.42 This evidence concerns the gene PRAME and cancer.