As expected, the results revealed that the L-kyn level was higher in the culture supernatant and EVs from IDO1- overexpression ovarian cancer cells than in those from IDO1-knockdown ovarian cancer cells or control ovarian cancer cells, indicating the aberrant upregulation of IDO1 in ovarian cancer cells contributed to the release of L-kyn-enriched EVs. This evidence concerns the gene IDO1 and ovarian carcinoma.