To determine the abundance of IL6 in HPV − and HPV + HNSCC, we established orthotopic tumors by intramucosal injection of HPV − mouse cancer MOC2 cells or HPV + mouse cancer TC-1 cells into C57BL/6 mice and then measured IL6 concentration in the peripheral serum extracted from these tumor models. This evidence concerns the gene IL6 and head and neck squamous cell carcinoma.