Our study showed that the proliferative, invasive and migratory abilities of ovarian cancer cells were inhibited after knockdown of ST14 protein, and those functions were restored by overexpression of TMEFF1 protein, suggesting that ST14 and TMEFF1 interact to form a protein complex, and ST14 can promote the proliferation, invasion and migration of ovarian cancer by regulating TMEFF1. This evidence concerns the gene TMEFF1 and ovarian cancer.