Remarkably, CD8+ CAR T cells activated at 39 °C showed (i) increased 3D motility in collagen gel (Fig. 8E and Supplementary Movies 53-56), an effect likely promoted by the increased mitochondrial activity since oligomycin abrogated it (Fig. 8E and Movies S53–56), and (ii) superior intratumoral motility when deposed onto viable tumor slices derived from the BxPC3-NSG model (Fig. 8F and Supplementary Movies 57, 58). Here, CD8A is linked to neoplasm.