The increased infiltration of Rapa-CD8CAR into the tumor mass was also confirmed by a competition assay upon injection of eFluor670 (eF670)-labelled ctrl-CD8CAR and CFSE-labelled Rapa-CD8CAR cells mixed in a 1:1 ratio into A549-derived subcutaneous tumor-bearing NSG mice, as shown by flow cytometry and immunofluorescence (Supplementary Fig. 10D). This evidence concerns the gene TRERF1 and neoplasm.