To explore the potential molecular biomarkers for the radiographic and pathologic response, we performed immunohistochemistry to detect PD-L1 expression, NGS to detect mutations of 437 cancer-related genes, and multiplex immunofluorescence to detect immune cell infiltration in pretreatment tumor biopsies from 47 patients and post-treatment surgical samples from 25 patients. This evidence concerns the gene CD274 and cancer.