Moreover, IF analysis using microglial markerIba-1, astrocyte marker GFAP and neuronal marker NeuN, showed that, compared with the sham group, neurons were significantly reduced, while microglia and astrocytes were significantly activated at 1 day after stroke, and this trend gradually recovered at 3 and 7 days after stroke (Fig. 1B), indicating that the animal model of AIS was successfully established and AIS could promote the inflammatory response and neuronal injury. Here, GFAP is linked to stroke disorder.