GFAP and Stroke: Moreover, IF analysis using microglial markerIba-1, astrocyte marker GFAP and neuronal marker NeuN, showed that, compared with the sham group, neurons were significantly reduced, while microglia and astrocytes were significantly activated at 1 day after stroke, and this trend gradually recovered at 3 and 7 days after stroke (Fig. 1B), indicating that the animal model of AIS was successfully established and AIS could promote the inflammatory response and neuronal injury.